Product: Anti-DEDD2 pAb
Cat. No.: PC-535 (100 μg)
Synonyms:
Anti-DNA-Binding Death Effector Domain-
Containing Protein 2, anti-FLAME-3.
Background:
Apoptotic signals are often triggered by cell
surface death receptors through protein-protein
interactions mediated by conserved domains
such as the death effector domain. A novel
death effector domain (DED)-containing protein,
DEDD2, has been recently identified and its
over-expression in transfected cells induces
moderate apoptosis and results in substantial
sensitization to apoptosis induced by Fas,
TRAIL, and FADD. More recently, work has
shown that DEDD2 can bind caspase-8 and -10
in addition to FLIP but not FADD. Like the
related protein DEDD, DEDD2 translocates from
the cytosol to the nucleus upon induction of
apoptosis, and it has been suggested that
DEDD2 may target caspase-8 to the nucleus
and that DEDD2 thus plays a critical role in
death receptor-induced apoptosis. At least two
alternatively spliced transcript variants encoding
distinct isoforms have been found for DEDD2.
Specificity:
Recognizes DEDD2.
Positive Controls:
RAW264.7 cell lysate.
Species Reactivity:
Human, mouse and rat. Other species not tested.
Host:
Rabbit
Immunogen:
A peptide corresponding to 11 amino acids near
the amino-terminus of human DEDD2 (GenBank
accession No. NP_579874).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 - 1 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of DEDD2 in
RAW264.7 cell lysate with anti-DEDD2 pAb at (A) 0.5
μg/mL, (B) 1 μg/mL, and (C) 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.