HA.11 Clone 16B12 Monoclonal Antibody, FITC Labeled-免疫学检测-试剂-生物在线
翌圣生物科技(上海)股份有限公司
HA.11 Clone 16B12 Monoclonal Antibody, FITC Labeled

HA.11 Clone 16B12 Monoclonal Antibody, FITC Labeled

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产品名称: HA.11 Clone 16B12 Monoclonal Antibody, FITC Labeled

英文名称: HA.11 Clone 16B12 Monoclonal Antibody, FITC Labeled

产品编号: FITC-101L

产品价格: 0

产品产地: Covance

品牌商标: Covance

更新时间: 2024-12-10T13:16:28

使用范围: null

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Description FITC Labeled Antibody, HA.11
Intended Use **Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use.
Clone 16B12
Form FITC Labeled Antibody (Stored in PBS + 0.03% Thimerosal + 50% Glycerol)
Host Mouse
IsoType IgG1
[Ab] 1 mg/mL
Specificity Monoclonal antibody HA.11 was raised against the twelve amino acid peptide, CYPYDVPDYASL. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. HA.11 recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

This antibody was subsequently purified via a protein-G column and conjugated to FITC.
Uses This antibody is effective in immunofluorescence (IF), immunoblotting, and immunoprecipitation (IP).
Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition.
  • Western blot: 1:1,000
  • IF: 1:1,000
  • IP: 1:150
Notes This product is also useful in western analysis when combined with our anti-FITC antibody (PRB-163P).
Storage This product contains 50% glycerol and should be stored at 4°C.
References Helliwell SB, Losko S, Kaiser CA. Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease. J Cell Biol 153(4):649-662, 2001.

Bennett BD, Denis P, Haniu M, Teplow DB, Kahn S, Louis JC, Citron M, Vassar R. A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's beta-secretase. J Biol Chem 275(48):37712-37717, 2000.

Kolodziej P, Young R. Epitope tagging and protein surveillance. Meth Enzymol 194:508-519, 1991.

Field J, Nikawa J, Broek D, MacDonald B, Rodgers K, Wilson I, Lerner R, Wigler M. Purification of RAS responsive adenylyl cyclase complex from Saccharomyces cervisiae by use of an epitope addition method. Mol Cell Biol 8:2159-2165, 1988.
Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.