FITC标记的磷酸化波形蛋白抗体
产品名称： FITC标记的磷酸化波形蛋白抗体
英文名称： Anti-Phospho-Vimentin (Ser56)/FITC
产品编号： HZ-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 IF=1:50-200
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Rabbit Anti-Phospho-Vimentin (Ser56)/FITC Conjugated antibody
FITC标记的磷酸化波形蛋白抗体
| 英文名称 | Anti-Phospho-Vimentin (Ser56)/FITC |
| 中文名称 | FITC标记的磷酸化波形蛋白抗体 |
| 别 名 | Vimentin (phospho S56); p-Vimentin (phospho S56); FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 发育生物学 神经生物学 信号转导 干细胞 细胞骨架 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 51kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Subunit: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain).Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Post-translational modifications: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Similarity: Belongs to the intermediate filament family. Database links: Entrez Gene: 7431 Human Entrez Gene: 22352 Mouse Entrez Gene: 81818 Rat Omim: 193060 Human SwissProt: P08670 Human SwissProt: P20152 Mouse SwissProt: P31000 Rat Unigene: 455493 Human Unigene: 691131 Human Unigene: 268000 Mouse Unigene: 2710 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Vimentin—波形蛋白。是五种主要的中间丝之一，存在于各种正常和病理性间质来源的组织，如纤维母细胞、内皮细胞、淋巴细胞等正常细胞和肉瘤、淋巴瘤、黑色素瘤等肿瘤。波形蛋白是负责维持细胞骨架完整性的蛋白之一 |
波形蛋白是间充质细胞中间丝的主要亚基蛋白。它被认为与细胞核和质膜之间蛋白质的胞内转运有关。Vimentin已经被牵涉到类固醇合成的速率，通过其作为甾体形成胆固醇的脂滴的存储网络的作用。波形蛋白磷酸化导致中间丝断裂和ATP和肌球蛋白轻链依赖性收缩事件的激活。这导致细胞骨架的变化，促进线粒体内脂滴的相互作用，以及胆固醇向细胞器的随后转运，从而导致类固醇合成的增加。免疫组化染色波形蛋白的特点是肉瘤（神经，肌肉和成纤维细胞来源）相比，癌通常是阴性的。黑色素瘤、淋巴瘤和血管瘤均可染色波形蛋白。波形蛋白抗体在鉴别未分化肿瘤和恶性肿瘤中具有重要价值。它们通常与一组其他抗体一起使用，包括识别细胞角蛋白、淋巴标记物、S100、结蛋白和神经丝。