Product: Anti-APG7 pAb
Cat. No.: PC-514 (100 μg)
Synonyms:
Anti-Autophagy Protein 7, anti-ATG7.
Background:
Autophagy, the process of bulk degradation of
cellular proteins through an autophagosomiclysosomal
pathway is important for normal
growth control and may be defective in tumor
cells. It is involved in the preservation of cellular
nutrients under starvation conditions as well as
the normal turnover of cytosolic components.
This process is negatively regulated by TOR
(Target of rapamycin) through phosphorylation
of autophagy protein APG1. Another member of
the autophagy family of proteins is APG7, which
was identified in yeast as an ubiquitin-E1-like
enzyme; this function is conserved in the
mammalian homolog. In mammalian cells,
APG7 is essential for autophagy conjugation
systems, autophagosome formation, starvationinduced
bulk degradation of proteins and
organelles. It has been suggested that caspase-
8 may alter APG7 levels and thus the APG7
program of autophagic cell death.
Specificity:
Recognizes APG7.
Positive Control:
Caco-2 cell lysate.
Species Reactivity:
Human and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
A 17 amino acid peptide from near the carboxyterminus
of human APG7 (GenBank Accession
No. NP_006386).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 - 1 μg/mL.

Immunocytochemistry: Use at ~10 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of APG7 in Caco-2
cell lysate with anti-APG7 pAb at (A) 0.5 μg/mL, (B) 1
μg/mL, and (C) 2 μg/mL.
Figure 2: Immunocytochemistry of APG7 in MCF7
cells with anti-APG7 pAb at 10 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product