SEPT2 抗体Applications
| In-house applications |
ELISA, IHC, IP, IF, WB, FC Application not tested? Please see our Guarantee. |
| Published applications |
IF, IP, WB |
Publications |
5 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
Prev12Next
 IF result from Dr. Kevin Corbit. SEPT2(11397-1-AP) marks the transition zone and cilium of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts. Immunofluorescent analysis of HepG2 cells, using SEPT2 antibody 113971-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). HeLa cells were subjected to SDS PAGE followed by western blot with 11397-1-AP(SEPT2 antibody) at dilution of 1:1000 IP Result of anti-SEPT2 (IP:11397-1-AP, 3ug; Detection:11397-1-AP 1:1000) with mouse brain tissue lysate 5000ug. 1X10^6 MCF-7 cells were stained with 0.2ug SEPT2 antibody (11397-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Immunohistochemical of paraffin-embedded human pancreas cancer using 11397-1-AP(SEPT2 antibody) at dilution of 1:50 (under 10x lens)
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| Positive WB detected in |
HeLa cells, C6 cells, human skeletal muscle tissue, Jurkat cells, K-562 cells, mouse brain tissue, mouse heart tissue, mouse testis tissue, rat brain tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human pancreas cancer tissue, human liver cancer tissue |
| Positive IF detected in |
HepG2 cells, hTERT-RPE1 cells and Mouse embryonic fibroblasts |
| Positive FC detected in |
MCF-7 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200 |
SEPT2 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information SEPT2 抗体
| Immunogen |
SEPT2 fusion protein ag1946 |
Full name |
Septin 2 |
Calculated molecular
weight |
41 kDa |
Observed molecular
weight |
45 kDa |
GenBank accession
number |
BC014455 |
Gene ID (NCBI) |
4735 |
| Gene symbol |
SEPT2 |
Synonyms |
DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, Pnutl3, SEPT2, septin 2, septin2 |
Background
|
SEPT2, also named as DIFF6, KIAA0158, NEDD5 and Septin-2, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. SEPT2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. SEPT2 is coordinated expression with SEPT6 and SEPT7. (PMID:21737677) SEPT2 抗体
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