低取代度硫酸葡聚糖
化学名称：Dextran, hydrogen sulfate sodium salt; Dextran sulphate; Sodium dextran sulfate; Dextran polysulfate
CAS 编号：9011-18-1
CAS 编号：9011-18-1
硫酸葡聚糖是由特定分子量的葡聚糖硫酸化反应制得的。TdB生产的低取代度硫酸葡聚糖分子量从5 kDa到500 kDa。
硫酸葡聚糖是以钠盐形式呈现，并加入少量磷酸盐作为稳定剂。 每个批次的产品都提供质检报告COA。严格仔细控制分子量程，含硫量，湿度等指标。
结构
葡聚糖是一种多糖，来源于肠系膜明串珠菌B512F，由α-D-(1-6)线性葡聚糖组成，侧链含量较低（约5%），与葡萄糖的碳-3位相连。硫酸葡聚糖是特定分子量的葡聚糖硫酸化得到的衍生物。低取代度硫酸葡聚糖的硫酸组分在8-13%之间。
图1。低取代度硫酸葡聚糖片段的结构表征。
硫酸葡聚糖（DS）可按不同平均重均分子量，不同分子量分布，不同程度的取代度，不同阳离子（例如钾替代钠）和不同硫酸化过程来生产。例如，仅凭硫酸葡聚糖70的名称无法完整识别该化合物，在评估这些化合物性能时应考虑到这一点。
硫酸葡聚糖是由选定的葡聚糖片段和硫酸化试剂（例如chlorosulfonic acid）合成而来，反应混合物经过纯化后，可以获得白色粉末状产品。
储存和稳定性
室温下，硫酸葡聚糖在干燥密闭的容器内可以保持稳定超过6年。
应用
硫酸葡聚糖涉及非常广泛的应用领域和性质。举例如下
- 细胞培养用的抗凝剂
- 脂蛋白的选择性淀析
- 加速DNA杂交
- 从DNA-组蛋白复合物中释放DNA
- 抑制tRNA与核糖体的结合
- 核糖核酸酶抑制剂
- 抗病毒性
- 用于化妆品的抗感染和透水保湿
- 分离微生物和大分子
- 疫苗佐剂
- 膜选择性渗透研究
产品列表
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产品编号
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品名
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分子量(kDa)
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包装
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Dextran sulfate 5 LS
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5
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10 g
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100 g
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|||
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Dextran sulfate 10 LS
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10
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10 g
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|
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100 g
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|||
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Dextran sulfate 20 LS
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20
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10 g
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|
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100 g
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|||
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Dextran sulfate 40 LS
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40
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10 g
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|
|
100 g
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|||
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Dextran sulfate 100 LS
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100
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10 g
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|
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100 g
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|||
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Dextran sulfate 500 LS
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500
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10 g
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|
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100 g
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参考文献
Möhwald, M. et al. Aspherical, Nanostructured Microparticles for Targeted Gene Delivery to Alveolar Macrophages. Adv Healthc Mater 6, ( 2017).
Dijk, M. et al. How Dextran Sulfate Affects C1-inhibitor Activity: A Model for Polysaccharide Potentiation. Structure 24, 2182–2189 (2016).
Shahraz, A. et al. Anti-inflammatory activity of low molecular weight polysialic acid on human macrophages. Scientific Reports 5, 16800 (2015).
Svensjö, E., Nogueira de Almeida, L., Vellasco, L., Juliano, L. & Scharfstein, J. Ecotin-Like ISP of L. major Promastigotes Fine-Tunes Macrophage Phagocytosis by Limiting the Pericellular Release of Bradykinin from Surface-Bound Kininogens: A Survival Strategy Based on the Silencing of Proinflammatory G-Protein Coupled Kinin B2 and B1 Receptors. Mediators of Inflammation https://www.hindawi.com/journals/mi/2014/143450/abs/ (2014) doi:10.1155/2014/143450.
Parraga, J. E., Zorzi, G. K., Diebold, Y., Seijo, B. & Sanchez, A. Nanoparticles based on naturally-occurring biopolymers as versatile delivery platforms for delicate bioactive molecules: An application for ocular gene silencing. International Journal of Pharmaceutics 477, 12–20 (2014).
Svensjö, E. et al. Maxadilan, the Lutzomyia longipalpis vasodilator, drives plasma leakage via PAC1–CXCR1/2-pathway. Microvascular Research 83, 185–193 (2012). Russo, L. M. et al. Renal Processing of Albumin in Diabetes and Hypertension in Rats. AJN 23, 61–70 (2003).
Landauer, K. et al. Influence of Carboxymethyl Dextran and Ferric Citrate on the Adhesion of CHO Cells on Microcarriers. Biotechnology Progress 19, 21–29 (2003). Hugerth, A. M. Micropolarity and Microviscosity of Amitriptyline and Dextran Sulfate/Carrageenan‐Amitriptyline Systems: The Nature of Polyelectrolyte–Drug Complexes. Journal of Pharmaceutical Sciences 90, 1665–1677 (2001).
Persson, B., Hugerth, A., Caram-Lelham, N. & Sundelöf, L.-O. Dextran Sulfate−Amphiphile Interaction; Effect of Polyelectrolyte Charge Density and Amphiphile Hydrophobicity. Langmuir 16, 313–317 (2000).
Hugerth, A. & Sundelöf, L.-O. Effect of Polyelectrolyte Counterion Specificity on Dextran Sulfate−Amphiphile Interaction in Water and Aqueous/Organic Solvent Mixtures. Langmuir 16, 4940–4945 (2000).
Burne, M. J. et al. Anomalous decrease in dextran sulfate clearance in the diabetic rat kidney. American Journal of Physiology-Renal Physiology 274, F700–F708 (1998).
Caram‐Lelham, N., Hed, F. & Sundelöf, L.-O. Adsorption of charged amphiphiles to oppositely charged polysaccharides—A study of the influence of polysaccharide structure and hydrophobicity of the amphiphile molecule. Biopolymers 41, 765–772 (1997).
Vyas, S. V., Burne, M. J., Pratt, L. M. & Comper, W. D. Glomerular Processing of Dextran Sulfate during Transcapillary Transport. Archives of Biochemistry and Biophysics 332, 205–212 (1996).
Wells, X. E. & Dawes, J. Role of the Liver and Kidney in the Desulphation of Heparin in vivo. Thromb Haemost 74, 667–672 (1995).
Vyas, S. V., Parker, J.-A. & Comper, W. D. Uptake of dextran sulphate by glomerular intracellular vesicles during kidney ultrafiltration. Kidney International 47, 945–950 (1995).
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