荧光素标记透明质酸
化学名称: 5-aminofluorescein-labelled hyaluronate; 5-aminofluorescein-labelled hyaluronan
商品名称: Fluorescein Hyaluronic acid (FHA-Se)
商品名称: Fluorescein Hyaluronic acid (FHA-Se)
透明质酸(HA)有多种各种来源，例如鸡冠或马链球菌。可提供用荧光素标记的这些来源的透明质酸衍生物（F-Ha）。检查所有批次的分子量、取代度、干燥失重和游离FITC。TdB实验室可以提供分子量约为6MDa的荧光素标记透明质酸。F-Ha为黄色粉末。
结构
透明质酸是一种源自马链球菌的由β（1-3）葡糖醛酸和β（1-4）氨基葡萄糖苷单元交替组成的多糖，使用5-氨基荧光素（氨基-四甲基罗丹明）标记得到黄色纤维状产品。该品溶于水和电解质，不过固体溶解时间长，需要在轻柔搅拌过夜（1）。取代度在0.001-0.008之间。标记过程不会导致透明质酸的解聚。分子量是由GPC系统检测，通过葡聚糖标准品矫校正，得到Mw（重均分子量）6.0 x106
图1 荧光素标记透明质酸分子结构示意图
光谱数据
荧光素标记透明质酸的最大激发波长 495 nm，最大发射波长524 nm。
图2. FITC标记透明质酸在pH9.0的0.025M硼酸盐缓冲液中 (12mg/50 ml缓冲液)的荧光吸收图谱。
激发波长495nm; 发射波长 524nm
储存和稳定性
按照de 干品应储存在密闭的容器中，室温下置于阴暗处。保质期建议为5年。当产品溶液在pH7.5，37°C下培养一个月后（1），未观察到荧光标记物质释放。
应用
在最近几年，透明质酸在医学（尤其对眼科手术不可或缺的作用）和化妆品中都有许多应用。荧光素标记的透明质酸可作为体外追踪透明质酸的探针。FITC标记透明质酸极大地增强了底物通过皮肤渗透的可视性（2）。荧光素标记透明质酸用于硅氧烷水凝胶材料的隐形眼镜对透明质酸的吸收研究。荧光素标记透明质酸的其他应用亦见诸报道（3-6）
产品列表
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产品编号
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品名
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分子量(kDa)
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包装
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Fluorescent hyaluronic acid
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6000
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100 mg
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参考文献
1. A.N. de Belder and K.O. Vik, Preparation and properties of fluorescein-labelled hyaluronate, Carbohyd. Res., 44(1975), 251-257.
2. J-E. Yang, E-S. Kim, J.H. Kwon et al., Transdermal delivery of hyaluronic acid – human growth hormone, Biomaterials, 33(2012), 5947-5954.
3. Y. Mitsui, M. Goto, T. Yamada et al., Hyaluronic acid inhibits mRNA expression of proinflammatory cytokines and cyclooxygenase-2/prostaglandin E2 production, Paper no. 209, 54th AM of Orthopaedic Society.
4. M. Yokoo, Y. Miyahayashi, T.Nagamuna, Identification of hyaluronic acid- binding proteins and their expressions in porcine cumulus-oocyte complexes during in vitro maturation, Biol Reprod, 67(2002), 1165-71.
5. D .Cheng, W. Han, K. Song et al., One-step facile synthesis of hylauronic acid functionalized fluorescent gold nanoprobes sensitive to hyaluronidase in urine specimen from bladder cancer patients, Talanta, 130(2014), 408-14.
6. M. Yoneda, S. Shimizu, Y. Nishi et al., Hyaluronic acid-dependent change in the extracellular matrix of mouse dermal fibroblasts that is conducive to cell proliferation, J Cell Sci., 90(1988), 275-286.
2. J-E. Yang, E-S. Kim, J.H. Kwon et al., Transdermal delivery of hyaluronic acid – human growth hormone, Biomaterials, 33(2012), 5947-5954.
3. Y. Mitsui, M. Goto, T. Yamada et al., Hyaluronic acid inhibits mRNA expression of proinflammatory cytokines and cyclooxygenase-2/prostaglandin E2 production, Paper no. 209, 54th AM of Orthopaedic Society.
4. M. Yokoo, Y. Miyahayashi, T.Nagamuna, Identification of hyaluronic acid- binding proteins and their expressions in porcine cumulus-oocyte complexes during in vitro maturation, Biol Reprod, 67(2002), 1165-71.
5. D .Cheng, W. Han, K. Song et al., One-step facile synthesis of hylauronic acid functionalized fluorescent gold nanoprobes sensitive to hyaluronidase in urine specimen from bladder cancer patients, Talanta, 130(2014), 408-14.
6. M. Yoneda, S. Shimizu, Y. Nishi et al., Hyaluronic acid-dependent change in the extracellular matrix of mouse dermal fibroblasts that is conducive to cell proliferation, J Cell Sci., 90(1988), 275-286.
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